Re: Normal Microbial Flora of the Human Body

On Sat, 6 Oct 2007 18:51:59 -0500, "Ralph" <nt_consulting64@xxxxxxxxx>
wrote:


"r norman" <r_s_norman@xxxxxxxxxxxx> wrote in message
news:jcgfg3tqllg4rr51sm0ct7957r4bsb0p9s@xxxxxxxxxx
On Sat, 6 Oct 2007 11:10:56 -0500, "Ralph" <nt_consulting64@xxxxxxxxx>
wrote:


"r norman" <r_s_norman@xxxxxxxxxxxx> wrote in message
news:t5afg3hddvpbusmp1ha5akc80gp3u737sl@xxxxxxxxxx
On Sat, 06 Oct 2007 14:03:07 -0000, evan <dr.lonlly@xxxxxxxxx> wrote:

Normal Microbial Flora of the Human Body see my site and tell me your
idea....


http://dr.lonlly.googlepages.com/normalmicrobialfloraofthehumanbody


You might also consider adding animals to make it Microbial Flora
and Fauna. For the curious onlooker, yes, there are animals that live
in and on us apart from disease causing parasites.


My favorite is Demodex mites. They can be easily found (eye-lash) and
examined under a good microscope. Guaranteed to keep a class of students
rubbing their eyes for a couple of days.


You say "easily". How do you do it? There's got to be a trick
because I have looked and looked specifically to use in into biology
labs. If you could just reveal the technique, I will put it to use!


You already have received good answers. The only thing I would add is that
it helps to have "oblique" lighting. If using a mirror you can simply play
with it, moving the focus point slightly off to the side and back again.
Surprisingly (in a way) if you are used to paramecium, et al. You will be
likely be surprised at how invisible they are comparatively.

Vary the light very carefully while you scan. As some one else noted it
helps when they move - but the movement isn't going to be dramatic. Once you
spot one - Bang! You'll have no trouble spotting them from then on.

As for eyelashes, I have had the most success with less trouble by merely
having students rub their eyes over white paper until a lash falls out.
(Works best in an Eight AM class. <g>) Then carefully mash and solve the
root along with the preparation the others have given you.


I am very familiar with using semi-dark field (or oblique
illumination from below) for viewing unstained transparent objects.
That is how I ordinarily illuminate my own specimens in neurobiology.
It tends to be very difficult with standard student lab microscopes.
It is even more difficult trying to explain how to do it to students!





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