Re: DNA sequencing
- From: Robert Weldon <rweldon@xxxxxx>
- Date: 26 Apr 2007 08:57:06 -0700
On Apr 25, 5:42 pm, "rmj" <gle...@xxxxxxx> wrote:
Does anyone know of any web site that goes into detail regarding the
methodology of sequencing DNA?
I can't imagine how the obstacles are overcome. The human chromosome is 3
billion or so base pairs. If one ended up with eight base pair fragments
there would be 4^8 (or almost 17 million) possible sequences; this means
close to 200 positions in the chromosome would have each combination. So one
would apparently need to work with even longer chains. In addition I suspect
that splitting the chromosome into smaller parts would be very unspecific.
In sequencing proteins one works with much smaller molecules with the
additional advantages that there are twenty-two amino acids and one can
create conditions of fairly specific cleaving.
Perhaps the scientific community is perpetrating a hoax; you know, like
staging the moon landing on a Hollywood set.
You have been educated on the DNA thing, so I will leave that. As for
the moon landing, I hope you were joking, because it was definitely
not a hoax.
.
- References:
- DNA sequencing
- From: rmj
- DNA sequencing
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