Re: Can Someone help with Agilent 5795 tuning issue.
Are they failing high or low. Do they fail after an autotune? If so:
What to look for
There is a lot of info on the Autotune report, which can make it
confusing, but we
can simplify it by focusing on what's important for us determine how
the system is
· First, look at the 3 principal peaks of the Autotune, 69, 219 and
their peak shapes and peak width. Peak shape should be smooth and
symmetrical and peak widths are typically in the 0.6 amu range.
· Next, look at the absolute abundance for ion 69. It should be in
the range of
approximately 1.5 million to 2.5 million counts for older systems and
For newer systems and software, the range is approximately 150,000 to
300,000 counts for the absolute abundance of ion 69.
· Relative to ion 69, ion 219 should be 35 percent or greater and 502
should be 2
percent or greater relative to the ion 69 absolute abundance.
· Isotope ratios will vary, but typically will be in the range of 1,
4, and 10 per cent
respectively, for ions 69, 219 and 502.
· Check the entrance lens offset parameter; it should be in the range
of 4.0 to 4.5
for systems that have a good vacuum, low background and a good clean
· Multiplier or EM voltage should be reasonable depending on EM age,
1400 and 2600 volts. The maximum voltage is 3000 volts.
more info (including what you see here) can be found on agilent's site: