Re: Recommendations for inexpensive microscope

On Tue, 29 May 2007 00:39:44 -0400, "JEDilworth"
<bactitech@xxxxxxxxxxxxxxxxxxxx> wrote:

Bacillus sp. are some of the largest bacteria.

Sp? Sorry, I don't know the lingo.

Bacteria are only a few
microns long in most cases. You would not be able to see them without
staining them. Unfortunately you would need an oil immersion lens to see
bacteria, regardless of the size.

Stain I understand (I remember some of this from way back). Oil
immersion...I'll have to check into that.

I believe Bacillus sp. are
approximately 8 microns long (at least B. anthracis is, but obviously
you wouldn't be looking at that one).

I hope not. It was apparently found on some animal skins last year
though. Someone making drum heads.

http://wizbangblog.com/2006/02/22/new-york-anthrax-case-not-terror-related-just-weird.php
http://www.wwdrums.com/anthrax-on-african-drums-faq-a-17.html

Bacteria are not identified by microscopic morphology per se, although
one of the first things we do when we're not sure what direction to go
in identification is to Gram stain the colony we're trying to identify.
Biochemical identification is how most clinical laboratories go about
identifying gram negative rods. There are other reactions used for ID of
gram positive cocci. These methods are too numerous and extensive to go
into here.

I do remember Gentian Violet for gram stains.

I've entertained the idea of getting a microscope for a while, but it
would have helped recently in diagnosing mites. I may as well get one
with as much capability as possible. I figured that bacteria would be
in range.

http://www.buddycom.com/bacteria/bacteria.html - this site has a lot of
good pics of bacteria. These are all probably taken with microscopes
with 100X oil immersion lenses.

100x is much less than I had assumed. Did you mean 1000x? (100x would
easily be in range of inexpensive microscopes, no?)

Just curious...how much magnification is usually required to see large
virii? I presume that's Electron Microscope territory.

Thanks for the links! I've bookmarked them.

Good luck. I really don't think you can do what you want to do without
spending some bucks.

What's the general range for the low end?
.

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