Re: Signal peptides sequences for E.coli

Julio wrote:

Hi,

my question is simple, i suppose, r there any list of signal peptides
recognised by E.coli (u use DH5alpha strains in expression exps) 'cause
I have to be sure that protein (with its signal sequence) from
Salmonella Enterica (still Enterobacteriaceae:)) will be processed in
the cytoplasm well and expedied to the periplasm as it is in nature.

Thanks for any help! Waiting for yours opinions

Signal peptides in E.coli using the sec system, as in any other gram negative bacterium, follow very simple rules outlined in Gunnar von Heijne's publications (they start with one or two positive residues, then a hydrophobic stretch follows, and then the signal peptide ends with two ala residues that is important for efficient peptide cleavage). I'm quite confident that a signal peptide from Salmonella would work efficiently in E.coli as long as it follows the rules for sec transport.

We have succesfully designed a new signal peptide based on these rules which was able to translocate recombinant proteins very efficient*, comparably to the commonly used signal peptides pelB and ompA. The results showed that the efficiency of the signal peptide is gene dependent, some signal peptides seem to work better with certain genes than others. I don't believe that the difference seen in measurable protein in the periplasm is necessarily caused by increased translocation, but rather by an increase in expression, probably translation.

*http://tinyurl.com/mscga

Trond Erik


--
Trond Erik Vee Aune
Institutt for Bioteknologi, NTNU
.

Relevant Pages

  • Signal peptides sequences for E.coli
    ... my question is simple, i suppose, r there any list of signal peptides ... recognised by E.coli (u use DH5alpha strains in expression exps) 'cause ... the cytoplasm well and expedied to the periplasm as it is in nature. ...
    (sci.bio.technology)
  • Signal peptides sequences for E.coli
    ... my question is simple, i suppose, r there any list of signal peptides ... recognised by E.coli (u use DH5alpha strains in expression exps) 'cause ... the cytoplasm well and expedied to the periplasm as it is in nature. ...
    (sci.bio.microbiology)